Virtual Basic Histocompatibility Course

Overview

Virtual Basic Histocompatibility Course, April 10-14, 2023 and April 17-21, 2023
Virtual Programs

The Virtual Histocompatibility Basic Course curriculum is designed to provide the technician/technologist with a well rounded background in basic transplant immunology and related laboratory skills and provides the transplant fellow with basic immunology.

Course presentations will be pre-recorded and available for one week with Q&A sessions on Friday, April 14 and Friday, April 21. To accommodate all schedules, both AM and PM live Q&A sessions will be held and recorded for future reference.

View Agenda

Pricing: $200

Online registration accepted only via Credit Card. Fees are non-refundable.

Contact Arlene Skinner with program questions, 804-323-9890, This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Objective

 

PURPOSE: The curriculum is designed to provide the technician/technologist with a well rounded background in basic transplant immunology and related laboratory skills and provides the transplant fellow with basic immunology.

 

OBJECTIVES: By the end of the course, the successful student will:

  1. Meet the needs of the beginning HLA technician/technologist by providing pertinent information about HLA testing, methodologies, and information necessary to meet the ASHI Statement of Competency.

 

SYLLABUS: Each student will receive a comprehensive course syllabus containing:

  • Lecture summaries
  • Lecture outlines
  • References and bibliography

 

CURRICULUM: Course presentations will be pre-recorded and available for one week with Q&A sessions on Friday, April 14 and Friday, April 21. To accommodate all schedules, both AM and PM live Q&A sessions will be held and recorded for future reference.

 

APPLICANT REQUIREMENTS: Technologist in Histocompatibility Testing, with a minimum (3) months experience in an HLA Laboratory is eligible OR a B.S. in an appropriate biological science or MT(ASCP) or other appropriate registry.

 

This course will have a limited number of registrants; early registration is recommended.

Call the AFDT Office at 804-323-9890 OR email Arlene Skinner at This email address is being protected from spambots. You need JavaScript enabled to view it. with program questions.

Week 1 Lectures

WEEK 1 PRE-RECORDED LECTURES (APRIL 10-14)

BASIC IMMUNOLOGY

(A. Hahn)

  • Innate immunity mediators
  • Adaptive immunity
  • Antibody structure, genetics, function
  • T cell receptor structure, genetics, function
  • MHC structure, polymorphism, inheritance
  • Antigen processing and presentation
  • T cell maturation, activation, effector functions
  • B cell development, activation
  • Antibody response—humoral immunity
  • Natural killer cell receptors, function

MOLECULAR TYPING

(M. Gautreaux)

  • Facilities
  • DNA extraction
  • Forward and Reverse SSOP
  • Sequence-specific Primers
  • Quantitative PCR/Real Time-PCR
  • Sanger Sequencing-Based Typing
  • Next Generation Sequencing Typing
  • QC & Troubleshooting

ALLELES, EPITOPES, HAPLOTYPES & DISEASE ASSOCIATIONS

(A. Hahn)

  • History of HLA
  • Protein and molecular terminology
  • CREGS, epitopes, eplets
  • Serology typing and crossreactivity
  • Polymorphism
  • Linkage Disequilibrium, haplotypes
  • Naming of HLA antigens/alleles
  • Null alleles, G and P groups, NMDP codes
  • Disease Associations
  • HLA-associated drug hypersensitivity

TRANSPLANT IMMUNOLOGY

(K. Hitchman)

  • Innate and adaptive mechanisms of rejection
  • KIR
  • Graft vs. Host Disease (GVHD)
  • ABO
  • Sensitization
  • Preventative and therapeutic immunomodulation (induction, maintenance, desensitization)
  • Immune memory/anamnestic responses

MOLECULAR GENETICS

(R. Liwski)

  • DNA Structure
  • RNA Structure and Function
  • Transcription and Translation
  • DNA Replication
  • MHC Gene Organization

LIVE Q&A SESSIONS (FRIDAY, APRIL 14)

10-11 AM AND 5-6 PM ET

Week 2 Lectures

WEEK 2 PRE-RECORDED LECTURES (APRIL 17-21)

SOLID ORGAN TRANSPLANTAITON

(M. Gautreaux)

  • History of transplantation
  • Kidney transplant overview
  • Other abdominal transplantation
  • Thoracic transplantation
  • Vascularized composite allografts
  • Organ allocation/UNOS/OPTN
  • Rejection

ANTIBODY DETECTION BY FLOW CYTOMETRY/LUMINEX

(K. Hitchman)

  • Principles of Flow Cytometry
  • Anti-HLA antibodies and transplantation
  • Evolution of antibody testing methods
  • Common current antibody testing methods
  • Sample considerations (serum selection, sample quality, timing, interference)
  • Analytics and interpretation
  • Pre-transplant vs. Post-transplant (DSA) considerations
  • Troubleshooting

HEMATOPOIETIC STEM CELL TRANSPLANTATION

(R. Liwski)

  • Autologous HSCT
  • Syngeneic HSCT
  • Allogeneic HSCT
  • HSC Sources
  • Donor Selection

CROSSMATCHING FOR TRANSPLANTATION

(M. Gautreaux)

  • Complement-Dependent Cytotoxicity
  • Flow Cytometric Crossmatching
  • Virtual Crossmatch
  • Renal vs. Non-Renal Crossmatching

QUALITY PROCESS & REGULATORY COMPLIANCE

(W. Herczyk)

  • Laboratory Quality Control
  • Laboratory Quality Assessment
  • Quality Improvement Plans
  • Laboratory Regulations/Compliance
  • Laboratory Safety

 

 

LIVE Q&A SESSIONS (FRIDAY, APRIL 21)

10-11 AM AND 5-6 PM ET

Registration

Please complete the form below to register for the Virtual Basic Histocompatibility Course. Registration is not complete until the payment is submitted.