Overview
Virtual Basic Histocompatibility Course, April 10-14, 2023 and April 17-21, 2023
Virtual Programs
The Virtual Histocompatibility Basic Course curriculum is designed to provide the technician/technologist with a well rounded background in basic transplant immunology and related laboratory skills and provides the transplant fellow with basic immunology.
Course presentations will be pre-recorded and available for one week with Q&A sessions on Friday, April 14 and Friday, April 21. To accommodate all schedules, both AM and PM live Q&A sessions will be held and recorded for future reference.
Pricing: $200
Online registration accepted only via Credit Card. Fees are non-refundable.
Contact Arlene Skinner with program questions, 804-323-9890, This email address is being protected from spambots. You need JavaScript enabled to view it.
Objective
PURPOSE: The curriculum is designed to provide the technician/technologist with a well rounded background in basic transplant immunology and related laboratory skills and provides the transplant fellow with basic immunology.
OBJECTIVES: By the end of the course, the successful student will:
- Meet the needs of the beginning HLA technician/technologist by providing pertinent information about HLA testing, methodologies, and information necessary to meet the ASHI Statement of Competency.
SYLLABUS: Each student will receive a comprehensive course syllabus containing:
- Lecture summaries
- Lecture outlines
- References and bibliography
CURRICULUM: Course presentations will be pre-recorded and available for one week with Q&A sessions on Friday, April 14 and Friday, April 21. To accommodate all schedules, both AM and PM live Q&A sessions will be held and recorded for future reference.
APPLICANT REQUIREMENTS: Technologist in Histocompatibility Testing, with a minimum (3) months experience in an HLA Laboratory is eligible OR a B.S. in an appropriate biological science or MT(ASCP) or other appropriate registry.
This course will have a limited number of registrants; early registration is recommended.
Call the AFDT Office at 804-323-9890 OR email Arlene Skinner at This email address is being protected from spambots. You need JavaScript enabled to view it. with program questions.
Week 1 Lectures
WEEK 1 PRE-RECORDED LECTURES (APRIL 10-14)
BASIC IMMUNOLOGY
(A. Hahn)
- Innate immunity mediators
- Adaptive immunity
- Antibody structure, genetics, function
- T cell receptor structure, genetics, function
- MHC structure, polymorphism, inheritance
- Antigen processing and presentation
- T cell maturation, activation, effector functions
- B cell development, activation
- Antibody response—humoral immunity
- Natural killer cell receptors, function
MOLECULAR TYPING
(M. Gautreaux)
- Facilities
- DNA extraction
- Forward and Reverse SSOP
- Sequence-specific Primers
- Quantitative PCR/Real Time-PCR
- Sanger Sequencing-Based Typing
- Next Generation Sequencing Typing
- QC & Troubleshooting
ALLELES, EPITOPES, HAPLOTYPES & DISEASE ASSOCIATIONS
(A. Hahn)
- History of HLA
- Protein and molecular terminology
- CREGS, epitopes, eplets
- Serology typing and crossreactivity
- Polymorphism
- Linkage Disequilibrium, haplotypes
- Naming of HLA antigens/alleles
- Null alleles, G and P groups, NMDP codes
- Disease Associations
- HLA-associated drug hypersensitivity
TRANSPLANT IMMUNOLOGY
(K. Hitchman)
- Innate and adaptive mechanisms of rejection
- KIR
- Graft vs. Host Disease (GVHD)
- ABO
- Sensitization
- Preventative and therapeutic immunomodulation (induction, maintenance, desensitization)
- Immune memory/anamnestic responses
MOLECULAR GENETICS
(R. Liwski)
- DNA Structure
- RNA Structure and Function
- Transcription and Translation
- DNA Replication
- MHC Gene Organization
LIVE Q&A SESSIONS (FRIDAY, APRIL 14)
10-11 AM AND 5-6 PM ET
Week 2 Lectures
WEEK 2 PRE-RECORDED LECTURES (APRIL 17-21)
SOLID ORGAN TRANSPLANTAITON
(M. Gautreaux)
- History of transplantation
- Kidney transplant overview
- Other abdominal transplantation
- Thoracic transplantation
- Vascularized composite allografts
- Organ allocation/UNOS/OPTN
- Rejection
ANTIBODY DETECTION BY FLOW CYTOMETRY/LUMINEX
(K. Hitchman)
- Principles of Flow Cytometry
- Anti-HLA antibodies and transplantation
- Evolution of antibody testing methods
- Common current antibody testing methods
- Sample considerations (serum selection, sample quality, timing, interference)
- Analytics and interpretation
- Pre-transplant vs. Post-transplant (DSA) considerations
- Troubleshooting
HEMATOPOIETIC STEM CELL TRANSPLANTATION
(R. Liwski)
- Autologous HSCT
- Syngeneic HSCT
- Allogeneic HSCT
- HSC Sources
- Donor Selection
CROSSMATCHING FOR TRANSPLANTATION
(M. Gautreaux)
- Complement-Dependent Cytotoxicity
- Flow Cytometric Crossmatching
- Virtual Crossmatch
- Renal vs. Non-Renal Crossmatching
QUALITY PROCESS & REGULATORY COMPLIANCE
(W. Herczyk)
- Laboratory Quality Control
- Laboratory Quality Assessment
- Quality Improvement Plans
- Laboratory Regulations/Compliance
- Laboratory Safety
LIVE Q&A SESSIONS (FRIDAY, APRIL 21)
10-11 AM AND 5-6 PM ET
Registration
Please complete the form below to register for the Virtual Basic Histocompatibility Course. Registration is not complete until the payment is submitted.